Technical Note: High-content imaging of brain-on-chip microfluidic devices using PreciScan intelligent acquisition.
Brain-on-chips (BoC) are innovative tools that simulate complex brain environments, enhancing our understanding of neurodegenerative diseases (NDDs). While a range of BoC platforms have been developed to model diverse NDD pathophysiologies, the various channel architectures of these microfluidic devices present challenges in image acquisition and analysis. High-content imaging protocols must be adaptable to these differing designs.
This Technical Note, developed by Revvity in collaboration with ETAP-Lab, demonstrates an automated image acquisition protocol for BoC microfluidic devices. The protocol utilizes the Operetta CLS™ high-content analysis system with PreciScan intelligent acquisition to effectively image neuronal cells in NETRI microfluidic devices.
Key highlights:
- How to automate image acquisition of microfluidic devices
- How to set up a pre-scan re-scan routine for selected relevant channels within brain-on- chip devices
- How to increase throughput for quantitative microscopy-based screening of brain-on-chip models
Contact our team if you would like proof-of-concept on your therapeutics using a compartmentalized microfluidic device with our protocols and expertise.
Download the Technical Note: https://www.revvity.com/fr-en/content/high-content-imaging-brain-chip-microfluidic-devices-using-preciscan-intelligent
